Curated Optogenetic Publication Database

Search precisely and efficiently by using the advantage of the hand-assigned publication tags that allow you to search for papers involving a specific trait, e.g. a particular optogenetic switch or a host organism.

Showing 26 - 27 of 27 results
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26.

Dynamic blue light-switchable protein patterns on giant unilamellar vesicles.

blue iLID in vitro Extracellular optogenetics
Chem Commun (Camb), 23 Jan 2018 DOI: 10.1039/c7cc08758f Link to full text
Abstract: The blue light-dependent interaction between the proteins iLID and Nano allows recruiting and patterning proteins on GUV membranes, which thereby capture key features of patterns observed in nature. This photoswitchable protein interaction provides non-invasive, reversible and dynamic control over protein patterns of different sizes with high specificity and spatiotemporal resolution.
27.

Blue Light Switchable Bacterial Adhesion as a Key Step toward the Design of Biofilms.

blue Magnets E. coli in vitro Control of cell-cell / cell-material interactions Extracellular optogenetics
ACS Synth Biol, 17 Aug 2017 DOI: 10.1021/acssynbio.7b00197 Link to full text
Abstract: The control of where and when bacteria adhere to a substrate is a key step toward controlling the formation and organization in biofilms. This study shows how we engineer bacteria to adhere specifically to substrates with high spatial and temporal control under blue light, but not in the dark, by using photoswitchable interaction between nMag and pMag proteins. For this, we express pMag proteins on the surface of E. coli so that the bacteria can adhere to substrates with immobilized nMag protein under blue light. These adhesions are reversible in the dark and can be repeatedly turned on and off. Further, the number of bacteria that can adhere to the substrate as well as the attachment and detachment dynamics are adjustable by using different point mutants of pMag and altering light intensity. Overall, the blue light switchable bacteria adhesions offer reversible, tunable and bioorthogonal control with exceptional spatial and temporal resolution. This enables us to pattern bacteria on substrates with great flexibility.
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